ÊɾúÌå±íÃæչʾÖƱ¸scFv

µ¥Á´¿¹Ì壨scFv£©ÊÇÓÉ¿¹ÌåÖØ¡¢ÇáÁ´µÄ¿É±äÇø»ùÒòͨ¹ýÒ»¶Î±àÂëÔ¼5-25¸öAAµÄÁ¬½ÓëÄ»ùÒò£¨linker£©Æ´½Óºó±í´ïÐγɵÄÖØ×éµ°°×¡£scFv¾ßÓзÖ×ÓÁ¿Ð¡¡¢×éÖ¯´©Í¸Á¦Ç¿¡¢ÌåÄÚÑ­»·°ëË¥Æڶ̡¢ÃâÒßÔ­ÐԵ͡¢Ò×ÓÚ½øÐлùÒò¹¤³Ì¸Ä½øµÈÓŵ㣬ÔÚ¼²²¡ÁÙ´²Õï¶Ï¡¢ÖÎÁÆ¡¢Ô¤·ÀµÈ·½Ãæ¾ßÓÐÖØÒª×÷Óá£ÀûÓÃÊɾúÌåչʾ¼¼Êõ£¬¿ÉÒÔÖƱ¸µÃµ½¸ßÇ׺ÍÁ¦¡¢½á¹¹Îȶ¨µÄscFv¿¹Ìå¡£±¾ÎĶԳ£ÓõÄscFvÊɾúÌåչʾϵͳ¼°scFvÊɾúÌ忹Ìå¿â¹¹½¨É¸Ñ¡×öÒ»½éÉÜ¡£

pCANTAB 5EÊɾúÌåչʾϵͳ

ÈËÃÇÀûÓÃÊɾúÌåչʾ¼¼ÊõÖƱ¸scFvÊɾúÌ忹Ìå¿â£¬¾­ÊɾúÌ忹Ìå¿âɸѡµÃµ½µÄÊɾúÌ忹Ìåͨ³£¾ßÓнϸߵÄÎȶ¨ÐÔ¡£pCANTAB 5EÊɾúÌåչʾϵͳÊÇscfvÊɾúÌå±íÃæչʾ×î³£ÓõÄϵͳ£¬¸ÃϵͳÓÉÊɾúÁ£ÔØÌ壨pCANTAB 5E£©¡¢ËÞÖ÷¾ú£¨´ó³¦¸Ë¾úTG1¡¢HB2151µÈ£©¡¢¸¨ÖúÊɾúÌåM13K07µÈ²¿·Ö×é³É¡£

ÊɾúÁ£ÔØÌ壺pCANTAB 5EÊÇscFvÊɾúÌå±íÃæչʾ×î³£ÓõÄÊɾúÁ£ÔØÌ壬´óСΪ4522bp£¬¾ßÌåͼÆ×¼°Ã¸ÇÐλµãÈçÏÂËùʾ£º

pCantab5EÊɾúÁ£ÔØÌåͼÆ×

ͼ1£ºpCANTAB 5EÊɾúÁ£ÔØÌåͼÆ×

ËÞÖ÷¾ú£ºpCANTAB 5EϵͳÖÐËÞÖ÷¾úÓд󳦸˾úTG1¼°´ó³¦¸Ë¾úHB2151Á½ÖÖ£¬ÊɾúÁ£ÇÖȾÁ½ÖÖËÞÖ÷¾ú»áµÃµ½Á½ÖÖ²»Í¬µÄ²úÎÇÖȾ´ó³¦¸Ë¾úTG1×îÖտɵõ½scfv-plllÈںϵ°°×£¬ÇÖȾ´ó³¦¸Ë¾úHB2151×îÖտɵõ½¿ÉÈÜÐÔµÄscFvµ°°×¡£Ô­ÒòÊÇÔÚÊɾúÁ£ÔØÌåµÄÄ¿µÄ»ùÒò²åÈëλµãÄ©¶ËÓÐÒ»¶ÎE-Tag¶ÌëÄ£¬¶ÌëĺóÓÐçúçêÖÕÖ¹ÃÜÂë×Ó£¬TG1¾úÖê¶ÔçúçêÖÕÖ¹ÃÜÂë×Ó¾ßÓÐÒÖÖÆÐÔ£¬µ°°×·­Òë¿Éͨ¹ýçúçêÖÕÖ¹ÃÜÂë¼ÌÐø·­ÒëÏÂÓεÄPlll»ùÒò£¬×îÖÕ²úÉúscfv-plllÈںϵ°°×£¬HB2151Ϊ·ÇÒÖÖÆÖê´ó³¦¸Ë¾ú£¬µ°°×µÄºÏ³ÉÔÚScFv»ùÒòºó¼´Í£Ö¹£¬×îÖÕ»áµÃµ½¿ÉÈÜÐÔµÄscFvµ°°×¡£

¸¨ÖúÊɾúÌ壺M13K07¸¨ÖúÊɾúÌåÊÇpCANTAB 5EÊɾúÌåչʾϵͳµÄÖØÒª×é³É²¿·Ö¡£ÊɾúÌåתÈëËÞÖ÷ϸ°ûºóÏëҪ˳Àû´«´ú±ØÐëÒªÓи¨ÖúÊɾúÌåµÄ²ÎÓë¡£

scFvÊɾúÌ忹Ìå¿â¹¹½¨¼°É¸Ñ¡

1. ÖƱ¸scFv»ùÒò¿â

ÊÕ¼¯Ä¿±ê¿¹ÌåËÞÖ÷µÄÃâÒßϸ°û£¨BÁÜ°Íϸ°û¡¢ÖÂÃôBÁÜ°Íϸ°û¡¢·ÇÖÂÃôBÁÜ°Íϸ°ûµÈ£©£¬ÌáÈ¡×ÜRNA£¬¾­RT-PCR·Ö±ðÀ©Ôö³ö¿¹ÌåµÄVHºÍVL±àÂë»ùÒò£¬´¿»¯»ØÊÕVHºÍVLƬ¶Î£¬Ê¹ÓÃLinkerÒýÎォȫÌ×VHºÍVL»ùÒòËæ»úÆ´½Ó³ÉscFv»ùÒò¿â¡£

VHºÍVLµÄÆ´½Ó·½·¨Ä¿Ç°ÓÐÁ½ÖÖ:

  • ½« LinkerÉè¼ÆÔÚ±í´ïÔØÌåÉÏ,Á½¶Ë¸÷ÓÐÏÞÖÆÐÔÄÚÇÐøøÇÐλµã¹©VHºÍVL²åÈë;
  • °Ñ LinkerµÄ±àÂëÐòÁзֱðÉè¼ÆÔÚÀ©ÔöVHºÍVLÒýÎïÖÐ,ÓÃPCRÖ±½ÓºÏ³ÉScFv»ùÒò£ºVHºÍVLͨ¹ý Linker»¥²¹ÐòÁÐ,»¥ÎªÒýÎï¼°Ä£°åºÏ³ÉÍêÕûµÄScFv»ùÒò,ÔÙÒÔÁ½¶ËµÄÒýÎï½øÐÐPCRÀ©ÔöµÃµ½´óÁ¿µÄScFv»ùÒò²úÎï¡£¸Ã·½·¨±»³ÆΪÖصþÑÓÉìÆ´½Ó·¨( splicing by overlap extension,SOE)

2. ½«scFv»ùÒòתÈëÊɾúÌå

½«scFv»ùÒòתÈëÊɾúÌåÄÚ£¬¿ÉÒÔÖ±½Ó½«scFv»ùÒòתµ½ÊɾúÌå»ùÒòÉÏ£¬Ò²¿ÉÒÔ½«scFv»ùÒòתÈëÊɾúÁ£ÔØÌåÖС£Í¨³£Ö±½Ó½«scFv»ùÒòתÈëÊɾúÌåÌåÄÚµÄЧÂʺܵͣ¬±È½Ï³£ÓõÄÊǵڶþÖÖ·½Ê½£¬½«scFv»ùÒòÁ¬½ÓÖÁpCANTAB 5EÔØÌåÉÏ£¬¹¹½¨ÊɾúÁ£¡£

3. ÇÖȾËÞÖ÷¾ú

ÊɾúÁ£¹¹½¨Íê³Éºó£¬±ã¿É½øÐÐËÞÖ÷¾úµÄÇÖȾ¡£ËÞÖ÷¾úÓд󳦸˾úTG1¼°´ó³¦¸Ë¾úHB2151Á½ÖÖ£¬Èç¹ûËÞÖ÷ΪTG1£¬×îÖյõ½scfv-plllÈںϵ°°×£¬²¢Õ¹Ê¾ÔÚÊɾúÌå±íÃæ¡£Èç¹ûËÞÖ÷ΪHB2151£¬×îÖյõ½scFvµ°°×£¬ScFvµ°°×±»Åŵ½Ï¸°ûÖÜÖʿռ䣬ÒòΪȱÉÙplll»ùÒò½á¹¹Óò¶ø²»ÄÜÁ¬½Óµ½ÊɾúÁ£¿ÅÁ£ÉÏ£¬¾­À©´óÅàÑø¿ÉÈÜÐÔ¿¹ÌåƬ¶ÎÔÚÖÜÖÊ¿Õ¼ä»ýÀÛ²¢ÒçÖÁÅàÑøÒºÖС£

ÊɾúÁ£×ªÈëËÞÖ÷¾úºó£¬¿ÉÔÚËÞÖ÷¾úÌåÄÚ×ÔÎÒ¸´ÖÆ£¬µ«ÓÉÓÚȱÉÙ±ØÒªµÄ¹¦ÄÜ»ùÒò£¬ÊɾúÁ£ÎÞ·¨ºÏ³Éµ¥Á´DNA¡¢ÎÞ·¨½øÐд«´ú¡£ÎªÁËʹÊɾúÌå˳Àû´«´ú£¬ÔÚËÞÖ÷¾úÉú³¤ÖÁ¶ÔÊýÉú³¤ÆÚºó£¬ÐèÒª½øÐи¨ÖúÊɾúÌ峬Ⱦ¡£¸¨ÖúÊɾúÌå¿ÉÒÔΪÊɾúÁ£Ìṩ±ØÒªµÄ¹¦ÄÜ»ùÒò£¨p¢òµ°°×¡¢°ü×°µ°°×µÈ£©£¬´ÙʹÖÊÁ£ºÏ³Éµ¥Á´DNA²¢Íê³É´«´ú¡££¨¸¨ÖúÊɾúÌ幤×÷Ô­Àí£©

4. »ñµÃscFv¿¹Ìå¿â

¸¨ÖúÊɾúÌ峬ȾËÞÖ÷¾úºó£¬ÊɾúÌ忪ʼ´«´ú²¢ÁѽâËÞÖ÷¾ú£¬ÅàÑø¹ýÒ¹ÀëÐÄÊÕ¼¯ÉÏÇå¼´¿É»ñµÃÊɾúÌåscFv¿¹Ìå¿â£»

5. ɸѡÊɾúÌ忹Ìå¿â£¬µÃµ½¸ßÇ׺ÍÁ¦µÄscFv

Ò԰е°°×Ϊ¹Ì¶¨Ï࣬ÒÔ¿¹Ìå¿âΪÁ÷¶¯Ï࣬¾­¹ýÒ»¶Îʱ¼äµÄ¹²Í¬·õÓýºó£¬Ï´È¥Î´½áºÏµÄÓÎÀëÊɾúÌ壬ȻºóÒÔ¾ºÕùÊÜÌåÏ´ÍÑÏÂÓë°Ð·Ö×Ó½áºÏÎü¸½µÄÊɾúÌ壬ϴÍѵÄÊɾúÌå¸ÐȾËÞÖ÷¾úºó¾­·±Ö³À©Ôö£¬½øÐÐÏÂÒ»ÂÖÏ´ÍÑ£¬¾­¹ý3ÂÖ¡«5Âֵġ°Îü¸½-Ï´ÍÑ-À©Ôö¡±ºó£¬¼´¿Éɸѡ³öÓë°Ðµ°°×¾ßÓиßÇ׺ÍÐÔÇҽṹÎȶ¨µÄscFvµ°°×¡£

¡¾ÍøÕ¾µØͼ¡¿¡¾sitemap¡¿

Á¢¼´Ñ¯¼Û
  • ÐÕÃû

  • µ¥Î»

  • µç»°

  • ×ÉѯÏîÄ¿

  • ÑéÖ¤Âë

  • ÁôÑÔ:

¡¾ÍøÕ¾µØͼ¡¿¡¾sitemap¡¿
¡¾ÍøÕ¾µØͼ¡¿¡¾sitemap¡¿