¿¹ÌåÈںϵ°°×

¿¹ÌåÈںϵ°°×£¨IgÈںϵ°°×£©ÊÇÖ¸ÔÚ»ùÒòˮƽÉϽ«Ä¿µÄ»ùÒòͬÃâÒßÇòµ°°×²¿·ÖƬ¶Î»ùÒòÏàÁ¬£¬²¢ÔÚÕæºË»òÔ­ºË±í´ïϵͳÖбí´ïµÄÖØ×éµ°°×¡£¿¹ÌåÈںϵ°°×¾ßÓп¹ÌåµÄÌØÐÔ¼°ÈںϹ¦Äܵ°°×µÄ»îÐÔ£¬¿É¹ã·ºÓ¦ÓÃÓÚÃâÒßÕï¶Ï¡¢ÃâÒßÖÎÁÆ¡¢¿¹Ìå´¿»¯¼°¿¹ÌåºÍ¿¹Ô­µÄ¶¨Á¿·ÖÎöµÈ£¬Ìرð¿ÉÓÃÓÚÃâÒßµ¼ÏòÒ©ÎïµÄÖƱ¸¡£¸ù¾Ý½áºÏµÄIgƬ¶ÎµÄ²»Í¬£¬¿ÉÒÔ½«¿¹ÌåÈںϵ°°×·ÖΪFabÈںϵ°°×¡¢FcÈںϵ°°×Óëµ¥Á´¿¹Ì壨scFv£©Èںϵ°°×¡£

½á¹¹

FabÈںϵ°°×¡¢µ¥Á´¿¹ÌåÈںϵ°°×
Ñо¿±íÃ÷£¬¿¹Ìå¿É±äÇøµÄN¶Ë¿Õ¼ä½á¹¹ÉÏÓ뻥²¹¾ö¶¨Çø£¨CDR£©ÐγɵĿ¹Ô­½áºÏ²¿Î»Ê®·Ö½Ó½ü£¬ÓеĿ¹Ìå¿É±äÇøN¶Ë²Ð»ùÉõÖÁÖ±½Ó²ÎÓ뿹ԭ½áºÏ²¿Î»µÄÐγɣ¬Èç¹û½«Ð§Ó¦µ°°×Ó뿹ÌåƬ¶ÎµÄN¶Ë½áºÏ£¬¿ÉÄܶԿ¹Ìå¿É±äÇøµÄ¿Õ¼ä¹¹ÐÍÔì³É½Ï´óÓ°Ï죬´Ó¶ø½µµÍ¿¹ÌåÓ뿹ԭµÄ½áºÏÄÜÁ¦¡£Òò´Ë£¬Í¨³£½«µ°°×Ó뿹ÌåƬ¶ÎµÄC¶Ë½øÐнáºÏ£¬Ðγɿ¹ÌåÈںϵ°°×¡£

FcÈںϵ°°×

FcÈںϵ°°×ÔڽṹÉÏÊǽ«¿¹ÌåµÄFcÇøÓ빦Äܵ°°×½øÐÐÈںϣ¬¿É½«FcµÄN¶Ë»òC¶ËÓëÄ¿µÄ»ùÒò½øÐÐÈںϡ£¸ù¾Ý½áºÏµ°°×µÄ²»Í¬£¬¿ÉÒÔÓжàÖÖ¹¹ÐÍ£¨ÈçÏÂͼ£©¡£

ͼ1£ºFcÈںϵ°°×µÄ¹¹ÐÍ

×÷ÓÃÔ­Àí

º¬Óп¹Ìå¿É±äÇøµÄ¿¹ÌåÈںϵ°°×
FabÈںϵ°°×ÓëscFvÈںϵ°°×º¬Óп¹ÌåµÄ¿É±äÇø£¬¿ÉÒÔ½øÐп¹Ô­-¿¹Ìå·´Ó¦£¬Æä×÷ÓÃÔ­ÀíΪÀûÓÿ¹Ìå-¿¹Ô­ÌØÒìÐÔ½áºÏµÄÌØÐÔ£¬Í¨¹ýÕâÖÖÌØÐÔµÄÒýµ¼£¬½«¾ßÓÐÉúÎï»îÐԵĵ°°×°ÐÏòÒýµ¼ÖÁϸ°ûµÄÌض¨²¿Î»£¬½ø¶ø·¢»ÓÒ»¶¨µÄÉúÎïЧӦ£¬Ò²¾ÍÊÇËùνµÄ¡°ÉúÎïµ¼µ¯¡±¡£

²»º¬¿¹Ìå¿É±äÇøµÄ¿¹ÌåÈںϵ°°×
¸ÃÀàÈںϵ°°×º¬ÓеĿ¹Ì幦ÄÜÇøΪFcÇø£¬²»ÄܽøÐп¹Ô­-¿¹Ìå·´Ó¦£¬Fc¶ÎµÄ×÷ÓÃΪÑÓ³¤Ò©ÎïÔÚѪ½¬ÄڵİëË¥ÆÚ¡¢Ôö¼ÓÈںϵ°°×µÄÎȶ¨ÐԵȡ£FcÈںϵ°°×Ò©Àí×÷Óõķ¢»ÓÒÀÀµÓÚ¹¦Äܵ°°×²¿·Ö£¬ÀûÓÃÊÜÌå-ÅäÌåÖ®¼äµÄÏ໥×÷ÓòúÉúһϵÁеÄÉúÎïѧЧӦ¡£

¿¹ÌåÈںϵ°°×ÖƱ¸

×î³õ¿¹ÌåÈںϵ°°×ÖƱ¸µÄ·½·¨Îª»¯Ñ§½»Áª·¨£¬µ«ÕâÖÖ·½·¨ÖƱ¸µÄ¿¹ÌåÈںϵ°°××é³É²»¾ùÒ»¡¢ÐÔÄܲ»Îȶ¨¡¢ÃâÒßÔ´ÐÔ´ó£¬Ëæ×Å»ùÒò¹¤³Ì¼¼ÊõµÄ·¢Õ¹£¬¸Ã¼¼ÊõÒѱ»ÌÔÌ­¡£Ä¿Ç°Ö÷ÒªÀûÓûùÒò¹¤³Ì¼¼ÊõÀ´½øÐп¹ÌåÈںϵ°°×µÄÖƱ¸¡£ÆäÖƱ¸Ô­ÀíΪ£º½«¿¹Ìå»ùÒòÓëÄ¿µÄµ°°×»ùÒòͨ¹ýÒ»¶Î½ÓÍ·ÐòÁУ¨linker£©½øÐÐÁ´½Ó£¬È»ºó½«Á´½Ó²úÎïÑÇ¿Ë¡ÖÁÔØÌåÖУ¬²¢ÓÃÔ­ºË»òÕßÕæºË±í´ïϵͳ½øÐбí´ï¡£

ÖƱ¸¿¹ÌåÈںϵ°°×¹ý³ÌÖУ¬Ò»¸ö¹Ø¼üµÄÎÊÌâÊÇÁ½µ°°×¼äµÄ½ÓÍ·ÐòÁÐ( Linker )µÄ³¤¶È£¬linkerµÄ³¤¶Ì¶Ôµ°°×ÖʵÄÕÛµþºÍÎȶ¨ÐԷdz£ÖØÒª¡£Èç¹û½ÓÍ·ÐòÁÐÌ«¶Ì£¬¿ÉÄÜÓ°ÏìÁ½µ°°×¸ß¼¶-½á¹¹µÄÕÛµþ£¬´Ó¶øÏ໥¸ÉÈÅ£»Èç¹û½ÓÍ·ÐòÁÐÌ«³¤£¬ÓÖÉæ¼°ÃâÒßÔ­ÐÔµÄÎÊÌâ¡£

¿¹ÌåÈںϵ°°×ÓëË«ÌØÒìÐÔ¿¹Ìå

¿¹ÌåÈںϵ°°×Êǽ«¿¹ÌåµÄ²¿·ÖƬ¶ÎÓëÄ¿µÄµ°°×½øÐÐÈںϱí´ïµÃµ½µÄÖØ×éµ°°×£¬Èô½«Á½¸ö¾ßÓв»Í¬¿¹Ô­ÌØÒìÐԵĿ¹ÌåƬ¶ÎÁ¬½ÓÖÁͬһµ°°×£¬¼´¿ÉµÃµ½Ë«ÌØÒìÐÔ¿¹Ìå¡£

µ¥¿Ë¡¿¹ÌåÓ뿹ÌåÈںϵ°°×Çø±ð

±í1£ºµ¥¿Ë¡¿¹ÌåÓ뿹ÌåÈںϵ°°×±È½Ï

Ïà¹Ø·þÎñ

Ïà¹ØÔĶÁ

• FcÈںϵ°°×µÄ½á¹¹ÓëÁÙ´²Ó¦ÓÃ