| Ũ¶È | 0.5 mg/mL |
| ÃâÒßËÞÖ÷ | Mouse |
| ÃâÒßÔ | DXd conjugated to keyhole limpet haemocyanin(KLH) |
| ÀàÐÍ | Monoclonal |
| ÑÇÐÍ | Mouse IgG1, ¦Ë |
| ´¿»¯·½·¨ | Purified by Protein A |
| ÌØÒìÐÔ | This antibody recognizes DXd and DXd structurally similar molecules. The antibody can bind to DS-8201. |
| ÑéÖ¤Ó¦ÓÃƽ̨ | ELISA |
| »º³åÒº | 1¡ÁPBS, pH7.4, containing 0.05% BSA, 50% glycerol and 0.03% Proclin 300. |
| ´¢´æ·½Ê½ | Shipped in ice bag. The antibody can be stored for 1-2 weeks at 2-8¡ãC. For long term storage, store it at -20¡ãC. Avoid repeated freezing and thawing cycles. |
| Ó¦ÓÃ | ELISA |
| ÍƼöÓÃÁ¿ | 0.1-2 ?g/mL |
The DXd conjugated Trastuzumab Sandwich ELISA assay is developed by using Mouse Anti DXd Antibody, mAb (Cat:DTA0311) and Mouse Anti-Human IgG Antibody[HRP], mAb as the capture and detection antibodies. The samples tested in this assay were DXd conjugated Trastuzumab.
Mouse Anti DXd Antibody, mAb (Cat:DTA0311) binds with DS-8201.
Coating antigen: DS-8201, 0.1 ?g
EC50=6.258 ng/mL.
Mouse Anti DXd Antibody, mAb (Cat:DTA0311) binds with BSA-DXd conjugate.
Coating antigen: BSA-DXd conjugate, 0.1 ?g/mL
Mouse Anti DXd Antibody, mAb (Cat:DTA0311) dilution start from 50 ng/mL.
EC50= 7.990 ng/mL.
DXd is a derivative of exatecan (DX8951f), a compound with increased activity and improved solubility compared to CPT, and described not to be an ABCC2 or ABCG1 substrate. DXd was used in several proprietary ADC programs, such as DS-8201a (Enhertu), U3-1402 and DS-6157a, conjugated at DAR8 and DS-1062a and DS-7300a conjugated at lower DAR (4) to limit their toxicity, that are either approved by the FDA (DS-8201a) or currently under clinical evaluation. Although the DXd payload presented lower passive membrane permeability than exatecan mesylate, it was found to be less myelotoxic and was therefore also selected for its improved safety profile.
The anti-payload antibody is a useful reagent in ADC research by quantifying and monitoring drug levels or accumulations in tumor tissue, and more. It can be used in PK, PD analysis and ELISA.
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